February Meeting

Join us this February 23 at 4:30pm to listen to our first presentation of the year followed by a networking session.

You will hear :
LeeAnn Chau Ramsay

Title: The spatial immune landscape of melanoma

Resume :
Melanoma is an immunogenic cancer with a high response rate to immune checkpoint inhibitors (ICIs). It harbors an elevated mutation burden compared to other cancers, and as a result, has abundant infiltrating lymphocytes within its microenvironment. Understanding the complex interplay between the stroma, tumor cells and distinct tumor infiltrating lymphocyte (TIL) subsets remains a significant challenge in immune oncology. To properly study this interplay quantifying spatial relationships of multiple cell types within the tumor microenvironment is crucial. To address this, we employed Cytometry Time of Flight (CyTOF) Imaging Mass Cytometry (IMC) to simultaneously quantify the expression of 35 protein markers, characterizing the microenvironment of 5 benign nevi and 67 melanomas. We profiled over 220,000 individual cells to identify melanoma, lymphocyte subsets, macrophage/monocyte and stromal cell populations, allowing for in-depth spatial quantification of the melanoma microenvironment. We found that within pre-treatment melanomas the abundance of proliferating antigen-experienced cytotoxic T cells (CD8+CD45RO+Ki67+) and their proximity to melanoma cells was associated with positive response to immunotherapy. Our study highlights the potential of multiplexed single cell technology to quantify spatial cell-cell interactions within the tumor microenvironment.

Research area : https://scholar.google.ca/citations?user=HHVqX1sAAAAJ&hl=en

Two ways to join the meeting :
via jitsi (link available on RSVP)
via YouTube Live: https://www.youtube.com/watch?v=3QYBYp46Zeg&t=88s

See you soon!
PS: If you want to present in a future event, contact us via Meetup or by email

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